Viral Vector Core Facility (La Jolla)

Getting Started

Internal SBMRI

  • If you have an iLab account, please login at the top right or use this link https://ilab.sanfordburnham.org/
  • If you do not have an iLab account, please click HERE to request an account. All fields are required.

 

External SBMRI

  • You must have a Customer ID and iLab account to request services from this facility.
  • To obtain a Customer ID please email Eveline Hernandez at ehernandez@sanfordburnham.org
  • To obtain an iLab account, please click HERE.


Forgot your password? Please click HERE.

 

Overview of Services

Our goal is to assist Sanford Burnham Medical Research Institute Scientists, as well as laboratories on a world-wide basis, with the production of high-quality, high-titer lentiviral and retroviral (and adenoviral) vectors at the lowest possible cost. The SBMRI viral vector core offers a variety of services including ready-to-transduce lentiviral (and adenoviral) particles, and custom lentiviral and retroviral packaging and titrations. Our brand new exciting “the works” service includes lentiviral vector construction for your constitutive/inducible cDNA/shRNA expression, viral packaging, and titration (ready-to-transduce control particles provided). In the near future, custom adenoviral productions including amplification, purification, and titration will be available.

Leadership

Alexey Terskikh, Ph.D. | Facility Director

(858) 646-3100 Ext. 5299

Location and hours of operation

Hours Location

Monday through Friday

10AM to 6PM

 

Building 7, Room 7112

Links and Resources

  1. External website http://sr.burnham.org/sr/homepage/Viral_Vectors/index.htm

 

Contacts

Name Role Phone Email Location
Chun-Teng Huang
Manager
 
(858) 646-3100 Ext. 4353
 
cthuang@sanfordburnham.org
 
La Jolla Campus
 

Services

Name Description Price
01. (SBMRI ONLY) Ready-to-Transduce Lentivirus Request    *
02. (SBMRI ONLY) Lentiviral Production Request    *
03. (SBMRI ONLY) Retroviral Production Request    *
04. (SBMRI ONLY) Adenoviral Production Request    *
05. (EXTERNAL ONLY) Ready-to-Transduce Lentivirus Request    *
06. (EXTERNAL ONLY) Lentiviral Production Request    *
07. (EXTERNAL ONLY) Retroviral Production Request    *
08. (EXT ONLY) Adenoviral Production Request    *
09. Biological Cell Culture Hood Request 

To request time on the cell culture hood, please send a request to the core.  Your time will be added to the calendar.

 
*
10. 100X viral concentration kit 

Each unit includes 3.5mL of solution A and 1mL of solution B, enough for 10mL viral supernatant concentration. Standard procedure will be given upon request. It's easy, fast, and cost-effective (50% less cost).

 
*
11. Serum-free VH media 

If you do lenti- or retro-viral production, considering our ready-to-use serum free virus harvest media to maximize your viral collection (up to three times). Our unit price is 25$ per 500mL which is 50% less cost than commercial available media.

 
*
12. Ready-to-use zbFGF 

If you use human basic FGF, considering our zebrafish basic FGF, a substitute for human bFGF on hEs cell culture. We have tested on hES, h/m NPC, and hGBM cells. Each unit equals to 1 micro gram of bFGF and we can provide the quantity you request.

 
*
1. Large scale DNA plasmid preparations     *
100X Viral Concentration Kit 

Each unit includes 3.5mL of solution A and 1mL of solution B, enough for 10mL viral supernatant concentration. Standard procedure will be given upon request. It's easy, fast, and cost-effective (50% less cost).

 
*
2. Ready-to-transduce lentiviral preps. (per vial)    *
3. Ready-to-use Polybrene (1 mg/mL, 100µL per aliquot, 5 aliquots)     *
Adeno Conc/purification    *
Adeno qual/quantity titration    *
Adeno Recovery    *
Biological Cell Culture Hood (per minute)    *
Biosafety level 2 virus shipping container    *
CMV-eGFP-CAAX (membrane localization eGFP)    *
CMV-mCherry-CAAX (membrane localization mCherry)    *
CMV-mCherry-CAAX-2A-NLS-eGFP<br /> (membrane localization mCherry and nuclear localization eGFP)    *
CMV-NLS-eGFP (nuclear localization eGFP)    *
CMV-NLS-mCherry (nuclear localization mCherry)    *
Concentrated virus packaging additional plate 

Each Plate

 
*
Concentrated virus packaging, with qualitative titration - 3 plates    *
Concentrated virus packaging, with qualitative titration - 6 plates    *
Concentrated virus packaging, with qualitative titration - 9 plates    *
Concentrated virus packaging, with quantitative titration (using FACS) - 3 plates    *
Concentrated virus packaging, with quantitative titration (using FACS) - 6 plates    *
Concentrated virus packaging, with quantitative titration (using FACS) - 9 plates    *
GIPZ-shRNA-scrambled <i>Open Biosystems</i>    *
hPGK-dTomato    *
hPGK-eGFP    *
hPGK-Luciferase    *
hPGK-mCherry    *
iPSC 4-in-1 reprogramming virus (hOct3/4, hSox2, hKlf4, hc-Myc) <i>Allele Biotechnology</i>    *
LKO.1-shRNA-scrambled (non-fluorescence vector) <i>Sigma-Aldrich</i>    *
Ready-to-use zbFGF 

If you use human basic FGF, considering our zebrafish basic FGF, a substitute for human bFGF on hEs cell culture. We have tested on hES, h/m NPC, and hGBM cells. Each unit equals to 1 micro gram of bFGF and we can provide the quantity you request.

 
*
Serum-free VH media 

If you do lenti- or retro-viral production, considering our ready-to-use serum free virus harvest media to maximize your viral collection (up to three times). Our unit price is 25$ per 500mL which is 50% less cost than commercial available media.

 
*
Step1: Ligation/transformation discontinue 

Step 1

 
*
Step2-1: Miniprep evaluation discontinue (PCR confirmation) 

Step 1 and 2-1

 
*
Step2-2: Miniprep evaluation discontinue (sequencing) 

Step 1, 2-1, and 2-2

 
*
Step2-3: Miniprep evaluation discontinue (transfection) 

Step 1 and 2

 
*
Step3: Maxi-prep discontinue 

Step 1, 2, and 3

 
*
Tet-inducible promoter-dTomato    *
Tet-inducible promoter-eGFP    *
The works: Custom constitutively expressed cDNA lentiviral vector - &lt; 1kb 

Insert size < 1kb

8 plates

 
*
The works: Custom constitutively expressed cDNA lentiviral vector - 1~2kb 

Insert size 1~2kb

8 plates

 
*
The works: Custom constitutively expressed cDNA lentiviral vector - 2~3kb 

Insert size 2~3kb

8 plates

 
*
The works: Custom constitutively expressed shRNA lentiviral vector - 1 shRNA 

1 shRNA

8 plates

 
*
The works: Custom constitutively expressed shRNA lentiviral vector - 2 shRNA 

2 shRNA (targeting the same gene)

9 plates

 
*
The works: Custom constitutively expressed shRNA lentiviral vector - 3 shRNA 

3 shRNA (targeting the same gene)

9 plates

 
*
The works: Custom inducible cDNA lentiviral vector - &lt; 1kb 

Insert size < 1kb

8 plates

 
*
The works: Custom inducible cDNA lentiviral vector - 1~2kb 

Insert size 1~2kb

8 plates

 
*
The works: Custom inducible cDNA lentiviral vector - 2~3kb 

Insert size 2~3kb

8 plates

 
*
Virus concentration and purification (per 135mL of unconcentrated soup)    *