Microarray/QPCR (La Jolla)

Getting Started

Internal SBMRI

  • If you have an iLab account, please login at the top right or use this link https://ilab.sanfordburnham.org/
  • If you do not have an iLab account, please click HERE to request an account. All fields are required.

 

External SBMRI

  • You must have a Customer ID and iLab account to request services from this facility.
  • To obtain a Customer ID please email Eveline Hernandez at ehernandez@sanfordburnham.org
  • To obtain an iLab account, please click HERE.


Forgot your password? Please click HERE.

 

Overview of Services

The facility provides microarray-based global analysis of gene expression in human, mice, and rats, using an Illumina platform.  Starting with investigator RNA samples, the facility confirms RNA quality, and then performs microarray analysis as well as initial data analysis.  Array data are returned to the investigator, and if desired, also passed on to the Informatics and Data Management facility for more detailed analysis.  Both mRNA and miRNA profiling are available.  Quantitative real-time PCR (Q-PCR) service is predominantly SYBR green-based analysis, but hybridization probe (e.g. TaqMan) approaches are supported.  Starting with RNA, the facility will utilize primers from investigators, or design custom primers and perform Q-PCR.   The facility provides control primers and assists with experimental design, as well as providing basic relative quantitation analysis. Q-PCR analysis in the facility can also provide gene, RNA, or viral copy number data.

Leadership

Kang Liu, PhD | Microarray/QPCR Specialist

(858) 646 - 3100 x3714

kangliu@sanfordburnham.org

 

Craig Hauser, PhD | Scientific Director

chauser@sanfordburnham.org

Location and hours of operation

Hours Location

Monday - Friday

9:00AM - 5:00PM

Excluding Holidays

Building 2 Room 2216

La Jolla, California 92103

 

Sanford-Burnham Medical Research Institute, La Jolla

10901 North Torrey Pines Road
La Jolla, California 92037

Links and Resources

  1. www.sanfordburnham.org
  2. Microarray/QPCR Public Website
  3. Microaray/QPCR Internal Website

Services

 

Microarray Request

In a typical experiment, a user would bring between 100 ng - 500 ng of total RNA for each array assay, and within about one week, receive analyzed expression data for 24,000-48,000 human or mouse genes. The IDM Shared Resource will assist with follow-up bioinformatic analysis, and interesting regulated gene targets can be rapidly validated by real-time PCR using the Q-PCR service.


Q-PCR Request

A user can bring several gene names or accession numbers and a few micrograms of total RNA for each sample to the Facility, and usually receive initial quantitative expression data within several days. Publication-ready quantitative analysis with sufficient replicates can often be obtained within a week. In addition to relative gene expression analysis, Q-PCR analysis in the Facility can also provide gene, RNA, or viral copy number data.