Flow Cytometry Shared Resource (La Jolla)

Getting Started

Internal SBMRI

  • If you have an iLab account, please login at the top right or use this link https://ilab.sanfordburnham.org/
  • If you do not have an iLab account, please click HERE to request an account. All fields are required.

 

External SBMRI

  • You must have a Customer ID and iLab account to request services from this facility.
  • To obtain a Customer ID please email Eveline Hernandez at ehernandez@sanfordburnham.org
  • To obtain an iLab account, please click HERE.


Forgot your password? Please click HERE.

 

Overview of Services

The facility provides access to high-speed cell sorting and analytical flow cytometry in two locations on the Sanford-Burnham campus.  Trained investigators have 24-hr/day access to a variety of analytical flow cytometers available for independent use.  Core staff provide technical expertise, hardware and software training, operate the facility’s cell sorters and are available to assist with analysis experiments for those who prefer to have their samples run by an expert cytometrist.  Scientists planning a flow cytometry experiment are encouraged to consult facility staff for assistance with protocols, fluorochrome selection or other aspects of experiment design.

 

Services

  • High speed cell sorting performed by facility personnel in two locations
    • Single-cell (clone) sorting into 96-well plates
    • Simultaneous sorting of up to 4 populations
  • Analytical flow cytometry: do-it-yourself 24hr/day, or assisted by appointment
  • Hardware and software training
  • Consultation
    • Experiment design
    • Data analysis and interpretation
    • Pre-publication manuscript review

 

Equpiment and Resources

 

  • LifeTechnologies Countess Automated Cell Counter

 

Analyzers

  • BD LSRFortessa 14-color FACS analyzer with HTS plate loader for 96 or 384-well plates and four solid state lasers (405 nm, 488 nm, 561 nm, 640 nm)
  • BD FACSCanto 6-color FACS analyzer with 488 nm and 633 nm lasers
  • BD FACSCalibur 4-color analyzer with 488 nm and 635 nm lasers

 

Sorters

  • BD FACSAria 12-color high-speed cell sorter with 407 nm, 488 nm, and 633 nm laser lines
  • BD FACSDiVa 7-color high-speed cell sorter with Argon (488 nm or 514 nm) Krypton (multi-line UV, 407 nm or 568 nm) and HeNe (633 nm) lasers

 

Computer Workstations

  • Computer workstations (Macintosh OSX) with large monitors and data analysis software packages including
    • FlowJo
    • ModFit LT
    • CellQuest

Price List

  SBMRI
Subsidized
SBMRI
Unsubsidized
External
Non-Profit
External
For Profit
Analyzers - independent use
(per hour)
$25 - $35 $31 - $44 $40 - $47 $65 - $91
Analyzers  - assisted use
(per hour)

unassisted rate

+ $30/hr

unassisted rate

+ $30/hr

unassisted rate

+ $30/hr

unassisted rate

+ $30/hr

Analyzer Training (per hour)

unassisted rate

+ $30/hr

unassisted rate

+ $30/hr

unassisted rate

+ $30/hr

unassisted rate

+ $30/hr

FACSDiVa Cell Sorter (per hour) $60.00 $75.00 $81.00 $154.80
FACSAria Cell Sorter (per hour) $72.00 $90.00 $97.20 $185.76

 

 

Leadership

Yoav Altman | Resource Director

yoav@sanfordburnham.org

Phone: 858-646-3100 x3569


Location and hours of operation

Hours Location

Staffed Mon - Fri, 9am - 6pm

 

Cytometers available 

24 hours a day/ 7 days a week

to trained users

 

Building 5, Room 5160 (Phone: 858-646-3739)

Building 12, Room 1113 (Phone: 858-646-3563)

La Jolla, California 92103

 

Sanford-Burnham Medical Research Institute, La Jolla

10901 North Torrey Pines Road
La Jolla, California 92037

Links and Resources

  1. SBMRI Flow Cytometry Public Webpage
  2. SBMRI Flow Cytometry Intranet Website

Schedulable Equipment and Resources

Analyzers (5)
Cell Sorting (2)